Practical How-To

General Resources

• eDNA Resources
• eDNA Society Manuals
• DNAquaNet Practical Guide
• 2nd National Workshop on Marine eDNA Primer
• Southern eDNA Society Resources Page
• Cold Spring Harbor Laboratory DNA Learning Center
• California Molecular Methods Workgroup
• Environmental DNA protocol development guide for biomonitoring
• Great Lakes Fishery Commission Science Transfer Toolkit

Sampling

• NatureMetric’s Practical Guide to DNA-based methods
• USGS eDNA Sampling Protocol
• Joint Nature Conservation Committee’s Protocol for Sampling Marine Benthic Sediment for DNA-based Analysis (JNCC Report No. 706)
• WSU Guidelines for Designing Environmental DNA Surveys for Target Species
• Genidaqs Field Collection Protocol

In the Lab

• CALeDNA Library Preparation
• MBON 12S Library Preparation

Sequencing

• Explore the Sequencing playlist on our YouTube channel

Software/Reference Database

• CALeDNA Data Exploration Resources: Google Earth Metadata for Sample Sites and the Open Tree of Life
• CALeDNA Metabarcoding Reference Database
• CALeDNA’s Anacapa software toolkit
• Cold Spring Harbor Laboratory’s DNA Subway for plant researchers
• Dada2
• Insect for assigning taxonomy
• QIIME2